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. 2020 Aug 25;37(11):2825–2838. doi: 10.1007/s10815-020-01929-w

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Fig. 4 — The proliferation analysis of leptin on stem/progenitor spermatogonia with WST-1 assay and xCELLigence RTCA. a The WST-1 assay data presents the proliferative effect of leptin on stem/progenitor spermatogonia in a dose- and time-dependent manner. Note that the maximum proliferative effect is observed in 100 ng/ml leptin treatment group on day 7 of culture, Mean ± SEM, n = 12. b The growth curve shows the dose-dependent proliferative effect of leptin on stem/progenitor spermatogonia compared with the control, in time, by xCELLigence RTCA. Analysis was performed real time for totally 7 days and results were expressed as a cell index (CI) value. c Statistical analysis of CI calculated from six repeated experiments, Mean ± SEM. d The EC50 of leptin was calculated approximately as 114 ng/ml based on the dose-response curves of cell index during 7 days by the RTCA software 1.2.1 (ACEA Bioscience)