Table 1.

Primer sequences used for real-time PCR performed on beagle gingival fibroblasts

Genes	Sense	Antisense
COL1A1	5'-CGGCTCCTGCTCCTCTTA-3'	5'-AACACGTTGCCGTTGTCG-3'
LOX	5'-TGCCAGTACCCAGAGGAGAG-3'	5'-CAGTCTATGTCTGCATTGTAGGTATCA-3'
ELN	5'-GTGTCTACAGGTGCGGTGGT-3'	5'-CTTGGCCTTGACTCCTGTTC-3'
MMP 1	5'-CAGGTTCTACATGCGCGTAA-3'	5'-TAGCACATCCTGACCCTGAA-3'
MMP 12	5'-GGACCCTGGTTATCCCAAA-3'	5'-TCCTTGGAAGAAATAGTAGTGTCTG-3'
TIMP 1	5'-TCACCAGAGAACCCACCAT-3'	5'-ATCTCATAACGCCGGTTTAAGT-3'
B2M	5'-TCCTCATCCTCCTCGCT-3'	5'-TTCTCTGCTGGGTGTCG-3'

PCR, Polymerase chain reaction; COL1A1, collagen type I alpha 1; LOX, lysyl oxidase; ELN, elastin; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; B2M, beta 2-microglobulin (canine housekeeping gene).