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. 2020 Nov 3;11(11):949. doi: 10.1038/s41419-020-03138-w

Fig. 3. MiR-671 facilitated PCa cells proliferation, migration, and invasion in vitro and in vivo.

Fig. 3

Colony formation assays (A), wound healing assays (B), and Transwell assays (C) were performed to assess the proliferation, migration, and invasion ability in miR-671 knockdown or overexpressing PC-3 cells. D In vivo PCa tumor formation in the xenograft male nude mouse models. E Final tumor weights were measured. F The xenograft tumor tissues were stained with H&E. G KI67 IHC staining in xenografts with LV-in-miR-671 cells or LV-in-NC cells. (H-left and I) The tail vein xenograft model was used to investigate the effects of miR-671 on PCa metastasis in vivo, and lung colonization ability was measured by bioluminescence imaging. (H, right) The lung sections were stained with H&E. Magnification, ×200. Scale bars, 100 μm. The data were presented as means ± SD from three biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; Student’s t-test.