Figure 4.
Parallel-stranded duplex enhances nuclease resistance. (A) (YGA)6 variants digested with DNase I at pH 7.5 or 5.5. DNase I preferentially cuts double-stranded DNA, suggesting that the parallel duplexes formed at pH 5.5 are resistant to DNase I degradation. (B) Shown are (YGA)6 variants digested with S1 nuclease at pH 7.5 or 4.5. Red labeled sequences correspond to full length DNA (18-nt), without any nuclease modification. Blue sequences represent the nuclease cleavage product without a terminal phosphate group. The magenta sequence corresponds to the nuclease cleavage product containing a terminal phosphate group, leading to increased mobility. To see this figure in color, go online.