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. 2020 Nov 3;10:18921. doi: 10.1038/s41598-020-75751-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time-course (30, 60, 120 and 240 min) expression of miRNAs showing significant differences in response to an oral lipid challenge in male C57BL/6 mice. Data are shown as mean ± SD; n ≥ 7. *p < 0.05, **p < 0.001, ***p < 0.0001, compared with t = 0 (control). (b) Relative expression of miRNAs determined 2 h after an oral lipid challenge given orally to male and female wild-type mice (grey), compared to controls (white); n ≥ 10 per group. Two-way ANOVA was followed by Bonferroni’s post-hoc tests for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001. (c) Heatmap of differentially expressed intestinal and hepatic miRNAs 2 and 4 h after an oral lipid challenge compared to controls (male mice); n ≥ 8 per group. Up- and down-regulated miRNAs compared to controls are represented in red and green, respectively.