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. 2020 Nov 3;10:18886. doi: 10.1038/s41598-020-76017-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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degU^Evo is responsible for the alteration in swarming motility and colony architecture. (a) Swarming motility assay of Ancestral, Evolved, degU^Anc, degU^Evo, ΔdegU, and Lab. LB plates fortified with 0.7% of agar were inoculated incubated for 16 h at 28 °C. Swarm expansion, resulting from bacterial growth, appears in white, whereas uncolonized agar appears in black. (b) Representative images showing the complex colony architecture development along with the indicated time points of the Ancestral and Evolved. The strains were grown in MSgg medium at 28 °C. (c) Representative images showing the complex colony architecture of the indicated strains on MSgg agar plates incubated for 96 h at 28 °C. Scale bars 1 cm. For all panels, the assays were repeated a minimum of three times. This figure was generated with Microsoft PowerPoint 2019 MSO (version 16.0.10366.20016; https://www.microsoft.com).