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. 2020 Nov 4;10:19049. doi: 10.1038/s41598-020-76122-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylated STAT-1 (P-STAT1 (Y701)) and STAT1 expression in primary airway cells in children with and without PARDS. Cells were gated on forward and side-scatter. Histograms of primary flow data along with cell counts are noted. Histogram data is scaled using the modal function in FlowJo analysis software (A). Boxplots of the mean fluorescence intensity (MFI) of P-STAT1 (B) and STAT-1 (C) along with the percent cells positive for P-STAT1 (D) or STAT1 (E) are shown. The fluorescence minus 1 (FM1) histogram was used to draw the percent positive gate for P-STAT1 and STAT-1, respectively. FM1 (gray histogram), No PARDS (circles/blue boxplot and blue histograms) and PARDS (squares/red boxplot) and red histograms). For all analyses, n = 19 (No PARDS), n = 15 (Yes PARDS). Two-tailed Mann–Whitney U test. *p < 0.05.