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. 2020 Nov 4;10:19018. doi: 10.1038/s41598-020-76038-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time-dependent changes in Tregs in cervical lymph nodes and spleen during periodontitis progression. (A) Representative data of CD4⁺ Foxp3⁺ cells in CD4⁺CD25⁺ gate with mean ± SD values. Foxp3 Mean Fluorescence Intensity (MFI), frequency, and the total number of Foxp3⁺ cells in CD4⁺ and CD4⁺CD25⁺ gates in (B) cervical lymph nodes and (C) spleen. *P < 0.05, **P < 0.01, ***P < 0.001.