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. 2020 Nov 4;10:19018. doi: 10.1038/s41598-020-76038-w

Figure 4.

Figure 4

IL-17A+ expression, and phenotypic and epigenetic changes of Tregs from of animals with periodontitis. (A) Gating strategy and representative data of IL-17A expression in Tregs from cervical lymph nodes with mean percentages ± SD values. (B) Analysis of the frequency and total number of IL-17A+ Tregs during experimental periodontitis. (C) Heat map with the Log2 fold-change mRNA expression of Treg and Th17-related genes in isolated Tregs from periodontitis-induced animals for ten days (pTregs) or controls (bTregs), each replica includes four pooled animal samples. (D) Percentage of methylation of 4 CpG sites in the CNS2 zone of the foxp3 gene in purified Tregs. (E) Original genomic sequence, and bisulfite conversion. FMO: Fluorescence minus one. *P < 0.05, **P < 0.01.