Figure 5.

Identification of genes/operons conferring 3-HP tolerance. Six selected genes/operons were individually deleted from E. coli W (A) and E. coli W ΔyieP (B). All the mutants were grown in the modified M9 minimal medium. The 300 mM 3-HP was supplemented to the culture medium when indicated. Symbols: E. coli W (wild-type and ΔyieP; black circles, solid line), ΔrydC (blue closed rectangles, dotted line), ΔydcA (green open diamonds, dotted line), ΔgadX (blue closed rectangles, solid line), ΔadiY (green closed diamonds, solid line), ΔykgEFG (blue crosses, solid line), and ΔyohJK (red closed diamonds, solid line).