Figure 5.

Real Time RT-PCR Amplification. After ~ 38 min (cycle 35 approx.) we observed an increase in the fluorescence when using Poly-NH₂-MNP magnetic nanoparticles, being. (a) Negative control, qRT-PCR mix was employed with just 8 µL of ultrapure. (b) Positive control, 8 µL of viral RNA from a commercial extraction kit were placed together with the qRT-PCR mixture. (c) Positive sample experiment implementing 10 µL of our Poly-NH₂-MNP magnetic nanoparticles instead of the ones from the qRT-PCR mixture coming in the commercial extraction kit. (d) The same extraction procedure was carried out as in (c) varying the amount of Poly-NH₂-MNP magnetic nanoparticles to 5 µL. Finally, the volume of magnetic nanoparticles (Poly-NH₂-MNP) employed in the qRT-PCR mixture was increased to 25 µL in (e) and 40 µL (f) accordingly. The observed amplification behavior remains relatively constant and independent of the Poly-NH₂-MNP magnetic nanoparticles concentration which highlights their performance.