Table 2.
Thermocycling qRT-PCR procedure established from a commercial IVD kit.
| Step | Temperature (°C) | Time (min) | Cycles |
|---|---|---|---|
| Retro transcription | 50 | 20 | 1 |
| Denaturation | 95 | 15 | 1 |
| Denaturation 2 | 94 | 0:15 | 40 |
| Amplification | 58 | 0:30 | |
| Cooling | 40 | 0:30 |
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