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. 2020 Nov 4;3:644. doi: 10.1038/s42003-020-01359-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Examples of positive MUC1-ST IHC staining in breast cancers (a negative example is included in (d)). b Summary of manual tissue scoring of MUC1-ST expression in breast cancers (n = 53 breast cancer cases). c CD163 manual scores in different regions of breast cancers (n = 53 breast cancer cases). d Examples of sequential sections stained for MUC1-ST (brown) and CD163 (brown) by IHC. e CD163 scores in different indicated regions of the tumour measured against MUC1-ST scoring (n = 53 breast cancer cases). Standard error of the mean shown and paired t test used for statistical analysis. Correlations were analysed using linear regression analysis (Pearson’s).