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. 2020 Nov 4;3:644. doi: 10.1038/s42003-020-01359-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Hierarchical clustering of all transcripts from matched MUC1-ST (n = 3 biologically independent samples) and MCSF (n = 3 biologically independent samples) treated monocytes after RNAseq and Partek flow analysis. b t-sne plot showing clustering of MUC1-ST and MCSF treated monocyte transcriptomes (n = 3). c Volcano plot showing the fold change and significance (FDR) of differentially expressed genes in matched MCSF and MUC1-ST macrophages (n = 3 biologically independent samples). Low expressed genes removed (see Supplementary Data 1; tab 3). d Top 50 differentially expressed genes between matched MUC1-ST and MCSF macrophages. Low expressed genes removed (see Supplementary Data 1; tab 3). e As (d) but bottom 50 expressed genes. f CXCL5 and SERPINE1 transcript expression in matched monocytes treated with MCSF (n = 3 biologically independent samples) or MUC1-ST (n = 3 biologically independent samples) for 7 days in serum-free media. g CXCL5 and PAI-1 protein levels in the supernatant of monocytes treated with MUC1-ST or MCSF for 7 days in serum-free media (n = 14 biologically independent samples). h CXCL5 and PAI-1 levels in the supernatant of monocytes treated with MUC1-ST or desialylated MUC1-ST (MUC1-T) for 7 days in serum-free media (n = 4 biologically independent samples). i CXCL5 (n = 8 biologically independent samples) and PAI-1 (n = 3 biologically independent samples) levels in the supernatant of MUC1-ST macrophages pre-treated with anti-Siglec-9 antibodies or isotype control. j CXCL5 levels in the supernatant of monocyte/T47D (±neuraminidase pre-treatment; NA) cocultures after 48 h of co-culture at a 5:1 ratio, n = 2 biologically independent samples with technical triplicate. k CXCL5 levels in the supernatant of monocyte/T47D or monocyte/T47D (core 2) cocultures after 48 h of co-culture at a 5:1 ratio. Representative of two independent experiments. Standard error of mean shown and paired t test used for statistical analysis. TPM transcripts per million.