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. 2020 Nov 4;3:644. doi: 10.1038/s42003-020-01359-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Neutrophil chemoattractant or associated factor transcript expression level in MUC1-ST macrophages (n = 3 biologically independent samples) and MCSF macrophages (n = 3 biologically independent samples). b Example FSC/SSC plots of primary neutrophils 48 h after being cultured in indicated media or supernatant (n = 5 biologically independent samples). ‘Live neutrophils’ were defined as live using a viability dye. c Numbers and phenotype of live neutrophils 48 h after being cultured in indicated media or supernatant (n = 5 biologically independent samples). ‘Live cells’ were defined as live using a viability dye. d Migration of neutrophils towards indicated media or supernatant over indicated time period (n = 5 biologically independent samples). e Heatmap showing differentially expressed extracellular matrix disassembly genes (GO:0022617) in MUC1-ST (n = 3 biologically independent samples) and MCSF (n = 3 biologically independent samples) macrophages. f MMP14 protein levels in supernatant of monocytes treated with indicated factors for 7 days (n = 13 biologically independent samples; desialylated MUC1-ST, MUC1-T, n = 8 biologically independent samples). g Number of neutrophils invading through basement membrane extract towards the indicated media or supernatant at the indicated time points (n = 5 biologically independent samples). h Number of breast cancer cells (MCF-7) invading through basement membrane extract towards the indicated media or supernatant at the indicated time points (n = 5 biologically independent samples). Standard error of mean shown and paired t test used for statistical analysis.