Table 2.

The protocol for making 7.9 kPa polyacrylamide gel.

Stiffness (Pa)	40% acrylamide (µl)	2% bisacrylamide (µl)	10 × PBS (µl)	diH2O (µl)	TEMED (µl)	1% APS (µl)	Total volume (µl)
7900	187.5	35	100	576.5	1	100	1000