Table 1.
List of yeast strains used in this study. For the genotypes, the standard nomenclature is used. Under “relevant genotype” the parental strains are indicated in bold. The open reading frames relevant for GalA utilization are underlined. The prefixes “p” and “t” denote promoters and terminators, respectively.
| Strain name | Relevant genotype | Source |
|---|---|---|
| CEN.PK2-1C | MATa leu2-3,112 ura3-52 trp1-289 his3-1 MAL2-8c SUC2 | EUROSCARF |
| EBY.VW4000 | CEN.PK2-1C Δhxt1-17 Δgal2 Δstl1::loxP Δagt1::loxP Δmph2::loxP Δmph3::loxP | Reference20 |
| JWY019 | MATa MAL2-8c SUC2 Δilv2 Δbdh1 Δbdh2 Δleu4 Δleu9 Δecm31 Δilv1 Δadh1 Δgpd1 Δgpd2 | Reference30 |
| SiHY001 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-AnGAR1-tENO1-pTDH3-HXT13-tSSA1-pTEF2-YlSDR-tADH1-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV040 | This study |
| SiHY002 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-TrGAR1-tENO1-pTDH3-HXT13-tSSA1-pTEF2-YlSDR-tADH1- pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV041 | This study |
| SiHY003 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-AnGAR1-tENO1-pTDH3-HXT13-tSSA1-pTEF2-SOR2-tADH1- pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV042 | This study |
| SiHY004 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-TrGAR1-tENO1-pTDH3-HXT13-tSSA1-pTEF2-SOR2-tADH1- pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV043 | This study |
| SiHY007 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pTDH3-HXT13-tSSA1-pTEF2-YlSDR-tADH1-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV046 | This study |
| SiHY008 | EBY.VW4000 Δura3::pCCW12-AnGATA-tPGK1-pTDH3-HXT13-tSSA1-pTEF2-SOR2-tADH1-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV047 | This study |
| SiHY030 | CEN.PK2-1C Δura3::pCCW12-AnGATA-pPGK1-AnGAR1 [R267L]-pTDH3-HXT13-pTEF2-SOR2-KanMX-tAgTEF; constructed from transformation with SiHV129 | This study |
| SiHY032 | CEN.PK2-1C Δura3::pCCW12-AnGATA-pPGK1-AnGAR1 [K261M, R267L]-pTDH3-HXT13-pTEF2-SOR2-KanMX-tAgTEF; constructed from transformation with SiHV128 | This study |
| SiHY062 | JWY019 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-AnGAR1 [R267L]-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV136 | This study |
| SiHY063 | JWY019 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-AnGAR1 [R267L, K261M]-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV137 | This study |
| SiHY072 | JWY019 Δura3::pCCW12-AnGATA-tPGK1-pPGK1-AnGAR1-pAgTEF-KanMX-tAgTEF; constructed from transformation with SiHV158 | This study |