Table 2.

A₇, A₈ and A₁₉ conformational exchange parameters from C1′ off-resonance R_1ρ relaxation dispersion experiments.

C1′ nucleotide	kex (s−1)		pE (%)		Δω (ppm)
	Method 1	Method 2	Method 1	Method 2	Method 1	Method 2
A7	2991 ± 693	3309 ± 357	0.62 ± 0.19	0.80 ± 0.08	4.16 ± 1.06	3.32 ± 0.16
A8	1580 ± 306	1994 ± 199	0.58 ± 0.09	0.59 ± 0.02	3.53 ± 0.51	3.42 ± 0.1
A19	2680 ± 456	2872 ± 205	1.20 ± 0.01	1.00 ± 0.05	3.31 ± 0.32	3.29 ± 0.09

The exchange parameters presented in this Table correspond to the population of the minor conformer (p_E), the rate exchange (k_ex) and the difference between the chemical shifts of major and minor conformers (Δω); they were inferred from individual fits of C1′ off-resonance relaxation dispersion data according to a classical, home-made protocol (Method 1, see “Materials and Methods”) or an approach developed by Al Hashimi’s group (Method 2). Experimental data were collected at 600 MHz, 25 °C and pH 6.5.