Table 3.
A19 conformational exchange parameters from off-resonance R1ρ relaxation dispersion experiments.
| A19 atom(s) | kex (s−1) | pE (%) | Δω (ppm) for C1’ | |||
|---|---|---|---|---|---|---|
| Method 1 | Method 2 | Method 1 | Method 2 | Method 1 | Method 2 | |
| C1′ alone | 2680 ± 456 | 2872 ± 205 | 1.20 ± 0.01 | 1.00 ± 0.05 | 3.31 ± 0.32 | 3.29 ± 0.09 |
| C1′, C8, C2 together | 2671 ± 459 | 3138 ± 228 | 1.20 ± 0.20 | 1.10 ± 0.08 | 3.31 ± 0.31 | 3.31 ± 0.09 |
The exchange parameters presented in this Table correspond to the population of the minor conformer (pE), the rate exchange (kex) and the difference between the chemical shifts of major and minor conformers (Δω); they were inferred from fits of off-resonance relaxation dispersion data according to Methods 1 or 2; the data are those collected from resonances of either C1′ alone or C1′, C8 and C2 together, at 600 MHz, 25 °C and pH 6.5.