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. 2020 Oct 22;8:593377. doi: 10.3389/fcell.2020.593377

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Copyright © 2020 Macrì and Di-Poï.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferation pattern and PC development in embryonic P. vitticeps cerebellum. (A–E) Double immunohistochemistry (IHC) staining for PCNA (green staining) and LHX1 (red) markers at various developmental stages, indicated as embryonic days post-oviposition (dpo), in the cerebellum of P. vitticeps. Arrowheads in panels (C–E) indicate the position of the incomplete fissure on the cerebellar pial surface. Insets in panels (B,C) show high magnifications of PC spatial organization. PS, pial surface; VS, ventricular surface; URL, upper rhombic lip. Scale bars: 100 μm.