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. 2020 Oct 22;8:593377. doi: 10.3389/fcell.2020.593377

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Copyright © 2020 Macrì and Di-Poï.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular characterization of GC patterning in B. fuliginosus. (A–E) Double IHC for PCNA (green staining) and PH3 (red) markers (A,B,D,E) or ISH for Atoh1 (C) at various indicated embryonic developmental stages between 15 and 30 dpo in the cerebellum of B. fuliginosus. Insets in panels (A,B,D,E) show high magnifications of mitotic progenitors on the pial surface. (F,G) Double IHC for PCNA (green) and SHH [(F); red] or ISH for Rora (G) at 40 dpo. The inset in panel (F) shows high magnification of SHH-positive PCs. PS, pial surface; EGL, external granule layer; IGL, internal granule layer; VS, ventricular surface; URL, upper rhombic lip; CP, choroid plexus. Scale bars: 50 μm.