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. 2020 Oct 22;8:594092. doi: 10.3389/fcell.2020.594092

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Copyright © 2020 González-Arranz, Gardner, Yu, Patel, Heldrich, Santos, Carballo, Jaspersen, Hochwagen and San-Segundo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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H2A.Z localization at chromosome ends requires telomere attachment to the nuclear envelope (NE). Immunofluorescence of spread pachytene nuclei from the swr1Δ mutant stained with DAPI to visualize chromatin (blue), anti-GFP to detect H2A.Z (green), and anti-Zip1 to mark the synaptonemal complex (SC) central region (red). White arrowheads mark the ribosomal DNA (rDNA) region lacking Zip1. Yellow arrows point to H2A.Z foci likely corresponding to the spindle pole body (SPB; see text). The cartoons on the left schematize the linker of the nucleoskeleton and cytoskeleton (LINC) complex and the status of telomeric attachment in the different situations analyzed. Scale bar, 2 μm. The strains are DP1182 (wild type), DP1280 (mps3-Δ2-64), and DP1305 (ndj1Δ). Twenty-six, 28, and 24 nuclei were examined for wild type, mps3-Δ2-64, and ndj1Δ, respectively.