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. 2020 Oct 22;11:590575. doi: 10.3389/fendo.2020.590575

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Copyright © 2020 Liu, Yang, Xie, Ling, Hu, Cao, Huang, Zhu and Hua

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) staining in the proximal tibia (A), and the results of semi-quantitative analysis (B). The activity of the osteoblast cell was significantly decreased, while the osteoclast cell was increased in diabetes mellitus (DM) and ketogenic diet (KD) groups. There was significantly lower expression of OCN and remarkably higher expression of TRAP in both DM and KD groups compared with the sham group (arrows refer to positive cells). “*” means P < 0.05 compared to sham group.