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. 2020 Oct 22;14:573422. doi: 10.3389/fncel.2020.573422

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Copyright © 2020 Jin, Chen, Wu, Zhang, Huang, Lin, Wang, Wang, Xu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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H₂S suppresses NH₄Cl-induced apoptosis of astrocytes. Panel (A) shows the immunostaining of astrocyte with Hoechst 33342 and Propidium Iodide (PI). Chromatin condensation in apoptotic cell is indicated by the red arrow, and necrotic cell stained in red is indicated by the green arrow, respectively. Cell apoptosis ratio (%) = The quantity of positive cells/The quantity of total cells. Panel (B) indicates the expression of cleaved caspase-3 in astrocytes treated with control, H₂S, NH₄Cl, and combination of H₂S and NH₄Cl, respectively. Panel (C) displays the expression of Bcl-2 and Bax in astrocytes treated with control, H₂S, NH₄Cl, and combination of H₂S and NH₄Cl, respectively. Data are presented as mean ± SEM of three independent experiments. *P < 0.05.