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. 2020 Oct 22;14:573422. doi: 10.3389/fncel.2020.573422

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Copyright © 2020 Jin, Chen, Wu, Zhang, Huang, Lin, Wang, Wang, Xu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of Nrf2 signaling by H₂S during NH₄Cl-induced toxicity in astrocytes. Panel (A) shows the expression of Nrf2 in the cytoplasm (left) and nucleus (right) with histograms on the top and typical Western blot pictures at the bottom. Panel (B) displays the immunostaining of Nrf2 and DAPI as well as merge pictures in astrocytes treated with control, H₂S, NH₄Cl, and combination of H₂S and NH₄Cl, respectively. Panel (C) indicates the expression of Nrf2 downstream genes HO-1 and GCLC treated with control, H₂S, NH₄Cl, and combination of H₂S and NH₄Cl, respectively, with typical Western blot picture on the left and histograms on the right. Data are presented as mean ± SEM of three independent experiments. *P < 0.05, and ^#P > 0.05.