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. 2020 Oct 22;14:573422. doi: 10.3389/fncel.2020.573422

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Copyright © 2020 Jin, Chen, Wu, Zhang, Huang, Lin, Wang, Wang, Xu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Nrf2 and H₂S on astrocytes with and without NH₄Cl treatment. Panel (A) shows that the antisense shRNA of Nrf2 attenuated the expression of Nrf2, HO-1, and GCLC in astrocytes with Western blot on the left and histogram on the right. Panel (B) shows the astrocyte viability, and panel (C) displays the lactate dehydrogenase (LDH) release in astrocytes treated with different combinations of control shRNA (nc-shRNA), antisense shRNA1 of Nrf2 (Nrf2-shRNA1), antisense shRNA2 of Nrf2 (Nrf2-shRNA2), H₂S, and NH₄Cl, respectively. Panel (D) indicates the cleaved caspase-3, and panel (E) shows the Bcl-2/Bax in astrocytes treated with different combinations of control shRNA (nc-shRNA), antisense shRNA1 of Nrf2 (Nrf2-shRNA1), antisense shRNA2 of Nrf2 (Nrf2-shRNA2), H₂S, and NH₄Cl, respectively, with Western blot at the bottom and histogram on the top. Panel (F) displays the immunostaining of Hoechst 33342 and PI in astrocytes under different cultures of control shRNA (nc-shRNA), antisense shRNA1 of Nrf2 (Nrf2-shRNA1), H₂S, and NH₄Cl, respectively, with staining picture on the top and histogram at the bottom. Data are presented as mean ± SEM of three independent experiments. *P < 0.05, and ^#P > 0.05.