Figure 2.

Identification of the region in KLHL2 that required for KLHL2-mediated ARHGEF7 protein degradation. A. Schematic representation of KLHL2 deletion mutants used in the study. B. 293T cells were co-transfected with Myc-ARHGEF7 and the indicated plasmids. Cell lysates were prepared and subjected to immunoprecipitation using the anti-FLAG antibody, followed by WB analyses with the indicated antibodies. C. Ectopically expressed the indicated plasmids promoted endogenous ARHGEF7 protein degradation. D. 293T cells were transfected with FLAG-ARHGEF7 and the indicated plasmids (Myc-KLHL2 WT, M1, M2, and M3) in dose-dependent manners. 24 h after transfection, cells were harvested for WB analyses. E. The quantification of immunoblot is shown in the lower panel. The mean values (S.D.) of three independent experiments are shown.