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. 2020 Oct 1;10(10):3328–3344.

Figure 2.

Figure 2

TP63 activates CEBPG by directly binding to its distal enhancer. A. The ATAC-Seq and indicated ChIP-Seq signals at CEBPG locus. The ATAC-Seq data of ESCC patients were downloaded from the TCGA database. The ChIP-Seq data of H3K27ac, TP63, and SOX2 of ESCC cell lines were obtained from GSE106563. B. The relative CEBPG mRNA expression following knockdown of TP63 in KYSE140 and TE5 cell lines. Data were shown as Mean ± SD. Unpaired t test. *, P<0.05; **, P<0.01; ns, not significant. C. Western blotting showing the expression changes of CEBPG and TP63 when silencing TP63 in KYSE140 and TE5 cell lines. D. Confirmation of the TP63 binding sites at the CEBPG locus by ChIP-qPCR. NC was used as a negative control. Unpaired t test. **, P<0.01; ns, not significant.