Figure 4.

Representative images of spatial relationship between tumor or stromal Zeb1+ cells and CD8+ TILs in triple negative breast cancers (double IHC labeling; brown, Zeb1; purple, CD8). A. Tumor with an area composed of cells highly expressing Zeb1 and low density of intratumoral CD8+ TILs (bottom), and an area composed of cells without Zeb1 expression, highly infiltrated by CD8+ TILs (up). B. Another tumor, with similar spatial relationship between Zeb1-expressing cells and CD8+ TILs as the one under A (Zeb1+ cells on the left and CD8+ TILs on the right). C. Tumor with no Zeb1 expression by tumor cells and high density of intratumoral CD8+ TILs. D. Tumor with two different areas: the area at the left contains a zone rich in Zeb1+ tumor cells which is devoid of CD8+ TILs even outside the Zeb1+ zone; the area at the right contains less Zeb1+ tumor cells and much higher density of CD8+ TILs. E. Tumor with stroma rich in Zeb1+ cells and high density of stromal CD8+ TILs. F. Tumor with no stromal Zeb1+ cells and with low density of both stromal and intratumoral CD8+ TILs. Bar =100 µm.