FIG 1.

Variation in phenotypic PI susceptibility of full-length Gag-protease from HIV-1 infected patient at different time points. (A) Sequences of the viral clones showing the amino acid changes in the MA, CA, P2, NC, p1, and p6 regions of Gag between baseline (pre-PI treatment) and viral failure (during PI treatment). (B) Full-length Gag-protease sequence was amplified from plasma samples and cloned into p8.9NSX+. VSV-G pseudotyped viruses encoding luciferase were produced by cotransfection in 293T cells. The PI susceptibility of pseudovirions derived from each patient was measured by luciferase activity, as determined using a single-replication-cycle drug susceptibility assay. Data are fold differences in IC₅₀s of LPV in comparison to that for the assay reference strain, p8.9NSX. Error bars represent standard errors of the means from at least three independent experiments performed in duplicate.