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Surface plasmon resonance analysis of glycan binding affinity of E. coli BL21 expressing wild-type Hia and Hia isogenic mutants
| Hia type | 2-3 SLN-Ac | 2-6 SLN-Ac |
|---|---|---|
| Hia wild type | 2.47 μM ± 0.8 | 110 nM ± 3.0 |
| Hia D618K | 842 nM ± 178 | 21 nM ± 1.0 |
| Hia A620R | 3.04 μM ± 0.7 | NBa |
| Hia D618K/A620 double | 3.42 μM ± 1.2 | NB |
NB, no binding using a OneStep injection at 10 μM; indicates a KD above 10 μM.
