Skip to main content
. 2020 Oct 1;13(10):2524–2533.

Figure 1.

Figure 1

MiR-451a is lowly expressed in stomach cancer cells and negatively regulates CAV1. (A) MiRNAs targeting CAV1 in the microT-CDS, StarBase, miRDB, TargetScan, and mirDIP databases were analyzed using the UpSetR package in R version 3.5.1. (B) The differences in the miR-451a mRNA levels in the stomach cancer tissues and adjacent normal tissues were analyzed either unpaired or paired using GraphPad Prism 8.2.1. (C) Schematic of the CAV1 3’UTR point mutation used in this study. (D) The regulatory relationship between miR-451a and CAV1 was analyzed by a dual-luciferase reporter assay. (E, F) After miR-451a was transfected either alone or with the CAV1 expression plasmid into HGC-27 or MKN-45 cells, the CAV1 expression was determined using RT-qPCR (E) and western blot (F). ns, not significant (P > 0.05); **P < 0.01; ****P < 0.0001, miR-451a vs miRNA NC; ####P < 0.0001, miR-451a + CAV1 vs miR-451a. ANT: adjacent normal tissues; miR-451a+CAV1: miR-451a was co-transfected with the CAV1 expression plasmid; CAV1: caveolin-1; miRNA NC: the negative control of miR-451a.