. 2020 Sep 9;7(Pt 6):985–994. doi: 10.1107/S2052252520011008

Table 1. Steady-state kinetic parameters for CO₂ hydration by native and V143I CA II.

	k _cat/K _m (µM ⁻¹ s⁻¹)	k _cat (µs⁻¹)	K _m (mM)	k ₁ ^† (M ⁻¹ s⁻¹)	k ₋₁ ^† (s⁻¹)	k ₂ ^† (s⁻¹)	k ₋₂ ^† (M ⁻¹ s⁻¹)	k ₃ ^† (s⁻¹)
Native	89 ± 7^‡/120^§	0.93 ± 0.05^‡/1.0^§	11 ± 1^‡	1.3 × 10⁸	1.8 × 10⁶	1.7 × 10⁷	2.0 × 10⁸	1.2 × 10⁶
V143I	11 ± 1^‡/9.3 ± 0.3^§	1.0 ± 0.2^‡/0.7 ± 0.1^§	100 ± 24^‡	—	—	—	—	—

^†

From Behravan et al. (1990 ▸).

^‡

From Fierke et al. (1991 ▸).

^§

From West et al. (2012 ▸).

Table 1. Steady-state kinetic parameters for CO2 hydration by native and V143I CA II.