FIGURE 4.

kLANA-mediated cellular transcriptional regulation. Total RNA was isolated from BJAB-mCherry, BJAB-kLANA and BJAB-rKSHV.219 cells and used for transcriptome analysis. The plotted expression levels are relative and normalized log₂ transformed values. For identifying differentially regulated genes the filtering criteria used were 1.5 fold change and a p-value less than 0.05. (A) Box plot for comparison of expression levels between kLANA bound and kLANA-free genes in BJAB-kLANA (left) or BJAB-rKSHV.219 (right) and BJAB-mCherry cells. Red boxes indicate kLANA-bound genes in kLANA positive cells (BJAB-kLANA or BJAB-rKSHV.219) and green boxes indicate the same genes in kLANA negative BJAB-mCherry cells. Gray boxes indicate genes not bound by kLANA. Boxes represent interquartile range with Min-Max whiskers. ***p < 0.001. (B) Volcano plots for differential regulation of all cellular genes in BJAB-kLANA (left) and BJAB-rKSHV.219 (right) cells. Green numbers or dots represent downregulated genes, red numbers or dots represent upregulated genes, and gray dots indicate genes that are not differentially regulated. The numbers of genes bound by kLANA at their TSS are marked in blue.