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. 2020 Oct 22;11:578943. doi: 10.3389/fphar.2020.578943

FIGURE 3.

FIGURE 3

BBR combined with Sta improves inflammatory status in KKAy mice. (A) Cytokine levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, monocyte chemotactic protein (MCP)-1, IL-6, C-reactive protein (CRP), and IL-10 in plasma were detected by ELISA. (B) Gene expression levels of IL-1β, TNF-α, MCP-1, IL-6, IL-10, and TLR4 in intestine were analyzed by qPCR. (C, D) Protein levels of TLR4, p38MAPK, p-p38MAPK, ERK1/2, p-ERK1/2, JNK, and p-JNK were determined by Western blot analysis. β-Actin was detected as the internal reference. Data are expressed as mean ± SEM, n = 10–12 for (A); n = four to five for (B–D). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Con. BBR, berberine; Con, control; Sta, stachyose.