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. 2020 Oct 22;10:1776. doi: 10.3389/fonc.2020.01776

Figure 1.

Figure 1

Mitochondrial fission factor (MFF) decreases mitochondrial activity and mass. (A) Evaluation of MFF overexpression. MCF7 cells, stably transduced with a lentiviral vector encoding for mitochondrial fission factor (MCF-MFF) or the empty-vector (MCF-7 Control), were subjected to protein extraction and immunoblotted for MFF. β-actin is shown as equal loading control. (B,C) MFF overexpression decreases mitochondrial activity and mass. Stably transduced MCF7 cells harboring MFF (MCF-MFF) and the respective empty-vector (MCF-7 Control) were seeded for 24 h and then mitochondrial activity and mitochondrial mass were quantitated by FACS analysis using the probes MitoTracker Orange (B) and MitoTracker Deep-Red (C). At least four replicates were performed in each experiment. Results are the average of the mean of three independent experiments and are expressed as percentages normalized to the control ± SEM. ***p < 0.001.