Table 2.
Significant changes in protein levels associated with oxidative phosphorylation in MCF7 cells over-expressing the mitochondrial fission factor (MFF).
| Symbol | Gene name | Fold change |
|---|---|---|
| Oxidative phosphorylation | ||
| ATP5A1 | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle | 9.846 |
| ATP5B | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide | 1781.432 |
| ATP5C1 | ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 | −2.919 |
| ATP5F1 | ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 | 2.216 |
| ATP5H | ATP synthase, H+ transporting, mitochondrial Fo complex subunit D | 1.623 |
| ATP5I | ATP synthase, H+ transporting, mitochondrial Fo complex subunit E | 1.736 |
| ATP5J | ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 | 1.673 |
| ATP5J2 | ATP synthase, H+ transporting, mitochondrial Fo complex subunit F2 | −2.37 |
| ATP5L | ATP synthase, H+ transporting, mitochondrial Fo complex subunit G | −15.581 |
| ATP5O | ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit | −601.813 |
| COX4I1 | Cytochrome c oxidase subunit 4I1 | 1.669 |
| COX4I2 | Cytochrome c oxidase subunit 4I2 | −137.623 |
| COX5A | Cytochrome c oxidase subunit 5A | 1.913 |
| COX5B | Cytochrome c oxidase subunit 5B | 2.364 |
| COX6B1 | Cytochrome c oxidase subunit 6B1 | 1.87 |
| COX6C | Cytochrome c oxidase subunit 6C | 2.38 |
| COX7A2 | Cytochrome c oxidase subunit 7A2 | −12.37 |
| COX7A2L | Cytochrome c oxidase subunit 7A2 like | 1.617 |
| CYB5A | Cytochrome b5 type A | 1.802 |
| CYC1 | Cytochrome c1 | 16.811 |
| CYCS | Cytochrome c, somatic | 1.821 |
| MT-ATP6 | ATP synthase F0 subunit 6 | −280.464 |
| MT-CO2 | Cytochrome c oxidase subunit II | 4.759 |
| MT-ND1 | NADH dehydrogenase, subunit 1 (complex I) | 11.786 |
| MT-ND2 | Mitochondrially encoded NADH dehydrogenase 2 | −7.366 |
| MT-ND5 | NADH dehydrogenase, subunit 5 (complex I) | −8.753 |
| NDUFA2 | NADH:ubiquinone oxidoreductase subunit A2 | 1.51 |
| NDUFA4 | NDUFA4, mitochondrial complex associated | 2.201 |
| NDUFA5 | NADH:ubiquinone oxidoreductase subunit A5 | 1.527 |
| NDUFA6 | NADH:ubiquinone oxidoreductase subunit A6 | −112.999 |
| NDUFA8 | NADH:ubiquinone oxidoreductase subunit A8 | 45.057 |
| NDUFA9 | NADH:ubiquinone oxidoreductase subunit A9 | 3.523 |
| NDUFA10 | NADH:ubiquinone oxidoreductase subunit A10 | 10.495 |
| NDUFA12 | NADH:ubiquinone oxidoreductase subunit A12 | −60.713 |
| NDUFA13 | NADH:ubiquinone oxidoreductase subunit A13 | 1.767 |
| NDUFAB1 | NADH:ubiquinone oxidoreductase subunit AB1 | −3.356 |
| NDUFB3 | NADH:ubiquinone oxidoreductase subunit B3 | 1.558 |
| NDUFB4 | NADH:ubiquinone oxidoreductase subunit B4 | 2.211 |
| NDUFB5 | NADH:ubiquinone oxidoreductase subunit B5 | −338.956 |
| NDUFB6 | NADH:ubiquinone oxidoreductase subunit B6 | 2.247 |
| NDUFB7 | NADH:ubiquinone oxidoreductase subunit B7 | −20.943 |
| NDUFB9 | NADH:ubiquinone oxidoreductase subunit B9 | 2.454 |
| NDUFB10 | NADH:ubiquinone oxidoreductase subunit B10 | 1.609 |
| NDUFS1 | NADH:ubiquinone oxidoreductase core subunit S1 | −77.83 |
| NDUFS2 | NADH:ubiquinone oxidoreductase core subunit S2 | −22.623 |
| NDUFS3 | NADH:ubiquinone oxidoreductase core subunit S3 | −21.085 |
| NDUFS5 | NADH:ubiquinone oxidoreductase subunit S5 | 1.686 |
| NDUFS6 | NADH:ubiquinone oxidoreductase subunit S6 | 1.977 |
| NDUFS7 | NADH:ubiquinone oxidoreductase core subunit S7 | −69.072 |
| NDUFS8 | NADH:ubiquinone oxidoreductase core subunit S8 | −45.818 |
| NDUFV1 | NADH:ubiquinone oxidoreductase core subunit V1 | 1.552 |
| NDUFV2 | NADH:ubiquinone oxidoreductase core subunit V2 | −5.91 |
| SDHA | Succinate dehydrogenase complex flavoprotein subunit A | 1.617 |
| SDHB | Succinate dehydrogenase complex iron sulfur subunit B | 2.018 |
| UQCR10 | Ubiquinol-cytochrome c reductase, complex III subunit X | 2.354 |
| UQCR11 | Ubiquinol-cytochrome c reductase, complex III subunit XI | 1.501 |
| UQCRB | Ubiquinol-cytochrome c reductase binding protein | 1.534 |
| UQCRC1 | Ubiquinol-cytochrome c reductase core protein I | −4.993 |
| UQCRC2 | Ubiquinol-cytochrome c reductase core protein II | 373.541 |
| UQCRH | Ubiquinol-cytochrome c reductase hinge protein | −40.845 |
| UQCRQ | Ubiquinol-cytochrome c reductase complex III subunit VII | 9.76 |
Fold change of proteins detected in MCF7-MFF vs. MCF7-Control cells. Red: Up-regulated proteins; Green: Down-regulated proteins. Proteomics was performed as described in Materials and Methods. Statistical analyses were performed using ANOVA and 1.5-fold-changes in proteins with a p < 0.05 were considered. Dataset containing proteins with significant altered expression profile were imported into the Ingenuity Pathway Analyses (IPA) software, which groups the differentially expressed proteins into known functions and pathways.