Table 3.

Significant changes in protein levels associated with the tricarboxylic acid cycle in MCF7 cells over-expressing mitochondrial fission factor (MFF).

Symbol	Gene name	Fold change
TCA cycle
ACO1	Aconitase 1	−33.921
ACO2	Aconitase 2	−14.648
CS	Citrate synthase	−34.112
DHTKD1	Dehydrogenase E1 and transketolase domain containing 1	−3.207
DLD	Dihydrolipoamide dehydrogenase	3.814
DLST	Dihydrolipoamide S-succinyltransferase	−3.202
FH	Fumarate hydratase	14.079
IDH1	Isocitrate dehydrogenase [NADP(+)] 1, cytosolic	4.014
IDH2	Isocitrate dehydrogenase [NADP(+)] 2, mitochondrial	−176.206
IDH3A	Isocitrate dehydrogenase 3 [NAD(+)] alpha	−17.146
IDH3B	Isocitrate dehydrogenase 3 [NAD(+)] beta	1.752
IDH3G	Isocitrate dehydrogenase 3 [NAD(+)] gamma	3.159
MDH1	Malate dehydrogenase 1	24.472
MDH2	Malate dehydrogenase 2	−211.122
OGDH	Oxoglutarate dehydrogenase	−47.17
OGDHL	Oxoglutarate dehydrogenase-like	4.76
SDHA	Succinate dehydrogenase complex flavoprotein subunit A	1.617
SDHB	Succinate dehydrogenase complex iron sulfur subunit B	2.018
SUCLA2	Succinate-CoA ligase ADP-forming beta subunit	3.127
SUCLG1	Succinate-CoA ligase alpha subunit	6.425

Fold change of proteins detected in MCF7-MFF vs. MCF7-Control cells. Red: Up-regulated proteins; Green: Down-regulated proteins. Proteomics was performed as described in Materials and Methods. Statistical analyses were performed using ANOVA and 1.5-fold-changes in proteins with a p < 0.05 were considered. Dataset containing proteins with significant altered expression profile were imported into the Ingenuity Pathway Analyses (IPA) software, which groups the differentially expressed proteins into known functions and pathways.