Table 4.
Significant changes in protein levels associated with glycolysis in MCF7 cells over-expressing mitochondrial fission factor (MFF).
| Symbol | Gene name | Fold change |
|---|---|---|
| Glycolysis | ||
| GLUT1 | Facilitated glucose transporter member 1 | −27.22 |
| HXK1 | Hexokinase 1 | −17.96 |
| HXK2 | Hexokinase 2 | –Infinity |
| HXK3 | Hexokinase 3 | 1.755 |
| ALDOA | Aldolase, fructose-bisphosphate A | −63.01 |
| ALDOC | Aldolase, fructose-bisphosphate C | 3.304 |
| ENO1 | Enolase 1 | −353.572 |
| ENO2 | Enolase 2 | −14.088 |
| ENO3 | Enolase 3 | 4.658 |
| FBP1 | Fructose-bisphosphatase 1 | −5.137 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 37.238 |
| GPI | Glucose-6-phosphate isomerase | 3.486 |
| PFKM | Phosphofructokinase, muscle | −5.855 |
| PFKP | Phosphofructokinase, platelet | –Infinity |
| PGAM1 | Phosphoglycerate mutase 1 | 3.86 |
| PGAM4 | Phosphoglycerate mutase family member 4 | 1.668 |
| PGK1 | Phosphoglycerate kinase 1 | 250.959 |
| PGK2 | Phosphoglycerate kinase 2 | −28.097 |
| PKLR | Pyruvate kinase, liver and RBC | −11.843 |
| PKM | Pyruvate kinase, muscle | −92.492 |
| TPI1 | Triosephosphate isomerase 1 | −44.171 |
| LDHA | Lactate dehydrogenase A | 2.82 |
| LDHA6LB | Lactate dehydrogenase A like 6B | −58.176 |
Fold change of proteins detected in MCF7-MFF vs. MCF7-Control cells. Red: Up-regulated proteins; Green: Down-regulated proteins. Proteomics was performed as described in Materials and Methods. Statistical analyses were performed using ANOVA and 1.5-fold-changes in proteins with a p < 0.05 were considered. Dataset containing proteins with significant altered expression profile were imported into the Ingenuity Pathway Analyses (IPA) software, which groups the differentially expressed proteins into known functions and pathways.