Table 6.
Significant changes in protein levels associated with the epithelial-mesenchymal transition (EMT), extracellular matrix and cytoskeleton in MCF7 cells over-expressing mitochondrial fission factor (MFF).
| Symbol | Gene name | Fold change |
|---|---|---|
| Epithelial-mesenchymal transition, ECM and cytoskeleton proteins | ||
| VIM | Vimentin | −122.836 |
| ACTA2 | Actin, alpha 2, smooth muscle, aorta | −1.639 |
| TNC | Tenascin C | −18.294 |
| CNN2 | Calponin 2 | 109.554 |
| CALU | Calumenin | 9.943 |
| CAND1 | Cullin associated and neddylation dissociated 1 | −50.592 |
| CANX | Calnexin | 289.77 |
| CFL1 | Cofilin 1 | −4.993 |
| CTNNA1 | Catenin alpha 1 | 15.851 |
| CTNNA2 | Catenin alpha 2 | −46.852 |
| CTNND1 | Catenin delta 1 | −50.914 |
| GSN | Gelsolin | −9.378 |
| KTN1 | Kinectin 1 | −27.16 |
| VCL | Vinculin | 2.965 |
| THBS1 | Thrombospondin 1 | −26.041 |
| TAGLN2 | Transgelin 2 | 3.578 |
| TAGLN3 | Transgelin 3 | −18.464 |
| CUL1 | Cullin 1 | −24.7 |
| CUL2 | Cullin 2 | −3.6 |
| CUL3 | Cullin 3 | −43.342 |
| CUL7 | Cullin 7 | −24.685 |
| CUL4A | Cullin 4A | −4.282 |
| CUL4B | Cullin 4B | −30.196 |
| DES | Desmin | −2.734 |
| COL11A1 | Collagen type XI alpha 1 chain | −156.421 |
| COL28A1 | Collagen type XXVIII alpha 1 chain | −65.077 |
| COL2A1 | Collagen type II alpha 1 chain | −110.781 |
| COL8A1 | Collagen type VIII alpha 1 | −7.542 |
| ACTB | Actin beta | −378.155 |
| ACTBL2 | Actin, beta like 2 | −100.94 |
| ACTC1 | Actin, alpha, cardiac muscle 1 | −101.765 |
| ACTG1 | Actin gamma 1 | 4.483 |
| ACTN1 | Actinin alpha 1 | −4.133 |
| ACTN2 | Actinin alpha 2 | −2.295 |
| ACT | Actin-like protein (ACT) gene | −20.682 |
| ACTG1P4 | Actin gamma 1 pseudogene 4 | −2.329 |
| ACTN4 | Actinin alpha 4 | 30.372 |
| MYH9 | Myosin, heavy chain 9, non-muscle | 820.527 |
| MYH10 | Myosin, heavy chain 10, non-muscle | 7.969 |
| MYH11 | Myosin heavy chain 11 | −46.952 |
| MYH7B | Myosin heavy chain 7B | −13.017 |
| MYL1 | Myosin light chain 1 | −10.816 |
| MYL6 | Myosin light chain 6 | 1.94 |
| MYL12A | Myosin light chain 12A | 2.173 |
| MYL6B | Myosin light chain 6B | –Infinity |
| MYO1B | Myosin IB | 1.551 |
| MYO1C | Myosin IC | −51.931 |
| TUBA8 | Tubulin alpha 8 | −15.245 |
| TUBA1A | Tubulin alpha 1a | 1.545 |
| TUBA3E | Tubulin alpha 3e | 1.5 |
| TUBA4A | Tubulin alpha 4a | −22.152 |
| TUBAL3 | Tubulin alpha like 3 | −3.393 |
| TUBB3 | Tubulin beta 3 class III | −3.965 |
| TUBB6 | Tubulin beta 6 class V | −18.369 |
| TUBB8 | Tubulin beta 8 class VIII | 1.78 |
| TUBB2A | Tubulin beta 2A class IIa | −2.783 |
| TUBB2B | Tubulin beta 2B class IIb | 2.353 |
| TUBB4A | Tubulin beta 4A class IVa | 2.216 |
| TUBGCP2 | Tubulin gamma complex associated protein 2 | –Infinity |
| TUBGCP6 | Tubulin gamma complex associated protein 6 | –Infinity |
| TUBA1B | Tubulin alpha 1b | −5.529 |
| TUBA1C | Tubulin alpha 1c | −11.125 |
| TUBB | Tubulin beta class I | −90.993 |
| TUBB4B | Tubulin beta 4B class IVb | −25.109 |
| DNM1 | Dynamin 1 | −20.927 |
| DNM2 | Dynamin 2 | −18.654 |
| DNM3 | Dynamin 3 | −9.012 |
| DNMBP | Dynamin binding protein | −4.552 |
| LAMA3 | Laminin subunit alpha 3 | −58.838 |
| LAMB2 | Laminin subunit beta 2 | −12.831 |
| LAMB3 | Laminin subunit beta 3 | −77.639 |
| LAMB4 | Laminin subunit beta 4 | −8.215 |
Fold change of proteins detected in MCF7-MFF vs. MCF7-Control cells. Red: Up-regulated proteins; Green: Down-regulated proteins. Proteomics was performed as described in Materials and Methods. Statistical analyses were performed using ANOVA and 1.5-fold-changes in proteins with a p < 0.05 were considered. Dataset containing proteins with significant altered expression profile were imported into the Ingenuity Pathway Analyses (IPA) software, which groups the differentially expressed proteins into known functions and pathways.