Table 7.
Significant changes in other protein levels in MCF7 cells over-expressing mitochondrial fission factor (MFF).
| Symbol | Gene name | Fold change |
|---|---|---|
| Other proteins | ||
| MTA1 | Metastasis associated 1 | −41.671 |
| MTA2 | Metastasis associated 1 family member 2 | −17.064 |
| MTSS1L | Metastasis suppressor 1 like | 2.028 |
| UCP3 | Uncoupling protein 3 | −62.533 |
| TIGAR | TP53 induced glycolysis regulatory phosphatase | −39.761 |
| TGFBI | Transforming growth factor beta induced | –Infinity |
| TGFBR3 | Transforming growth factor beta receptor 3 | −8.57 |
| TGIF2LX | TGFB induced factor homeobox 2 like, X-linked | −12.818 |
| STAT1 | Signal transducer and activator of transcription 1 | −9.228 |
| STAT3 | Signal transducer and activator of transcription 3 | −7.953 |
| OXSR1 | Oxidative stress responsive 1 | 6.331 |
| PCNA | Proliferating cell nuclear antigen | 3.128 |
| HSF1 | Heat shock transcription factor 1 | −28.884 |
| TRAP1 | TNF receptor associated protein 1 | −10.989 |
| CAT | Catalase | −10.918 |
| SOD2 | Superoxide dismutase 2, mitochondrial | −33.389 |
| TXNRD1 | Thioredoxin reductase 1 | −21.824 |
| GLRX3 | Glutaredoxin 3 | −1.845 |
| GSR | Glutathione-disulfide reductase | −1E+08 |
| GSS | Glutathione synthetase | 2.547 |
| GSTM3 | Glutathione S-transferase mu 3 | 18.065 |
| ROMO1 | Reactive oxygen species modulator 1 | 1.966 |
| SMAD9 | SMAD family member 9 | −1.975 |
| TXNL1 | Thioredoxin like 1 | −9.869 |
| TXNRD2 | Thioredoxin reductase 2 | −3.538 |
| TXNRD3 | Thioredoxin reductase 3 | –Infinity |
| TMX1 | Thioredoxin related transmembrane protein 1 | 3.142 |
| TMX4 | Thioredoxin related transmembrane protein 4 | 1.518 |
| TNFRSF12A | TNF receptor superfamily member 12A | –Infinity |
| TNFSF13B | Tumor necrosis factor superfamily member 13b | 2.397 |
| TRAF2 | TNF receptor associated factor 2 | −7.759 |
Fold change of proteins detected in MCF7-MFF vs. MCF7-Control cells. Red: Up-regulated proteins; Green: Down-regulated proteins. Note down-regulation of relevant proteins involved in the metastatic process and oxidative stress response. Proteomics was performed as described in Materials and Methods. Statistical analyses were performed using ANOVA and 1.5-fold-changes in proteins with a p < 0.05 were considered. Dataset containing proteins with significant altered expression profile were imported into the Ingenuity Pathway Analyses (IPA) software, which groups the differentially expressed proteins into known functions and pathways.