Fig. 5.

Pathology scoring of tubular damage in C57BL/6 mice treated with repeated administration of 9 mg/kg cisplatin. Hematoxylin and eosin (H&E)-stained and periodic acid-Schiff (PAS)-stained sections were scored on a scale of 1–4 for severity of loss of brush border (A), tubular casts (B), tubular dilation (C), tubular degeneration (D), widening of the interstitium (E), inflammation (F), tubular necrosis (G), and tubular regeneration (H) by a mouse renal pathologist. Statistical analysis was determined by individual chi-square tests. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. I and J: representative H&E-stained sections. Reg, regeneration; c, cast; I, inflammatory cells; n, necrosis; Wi&I, widening of the interstitium and inflammatory cells.