Table 1.
Comparison of traits among human mesothelial cell lines
| Characteristic | HMC Primary | NP Cells | MeT-5a | LP-9 |
|---|---|---|---|---|
| Nonadherent growth | No (5) | No* | Yes (36) | No (28) |
| Cell surface microvilli | Yes (1) | Yes* | Yes (7) | Yes (39) |
| Mesothelial markers (CDH1, MSLN) | CDH1 (23) | CDH1* MSLN* |
CDH1 (47) MSLN (12) |
MSLN (17) |
| Karyotype | Normal (11) | Normal* | Hyperploidy (41) | Normal (48) |
| Senescence limit | ~14 wk (9) | hTERT immortalized* | SV40 immortalized (20) | ~14 wk (24) |
| Mesothelial-specific cytokine profile | ||||
| IL-6 | Yes (35) | Yes* | Yes* | Yes (15) |
| IL-8 | Yes (46) | Yes* | Yes* | Yes (40) |
| GCSF | Yes (25) | Yes* | No* | Yes (16) |
| VEGF | Yes (45) | Yes* | No* | Yes (16) |
| MCP-1 | Yes (2) | Yes* | No* | Not reported |
| PAI-1 | Yes (49) | Yes* | No* | Not reported |
| Intercellular tight junctions | Yes (18) | Yes* | Yes (42) | Not reported |
HMC, human mesothelial cell; NP, normal pleura; CDH1, cadherin 1; MSLN, mesothelin; hTERT, human telomerase reverse transcriptase; SV40, simian virus 40; GCSF, granulocyte colony-stimulating factor; VEGF, vascular endothelial growth factor; MCP-1, monocyte chemoattractant protein-1; PAI-1, plasminogen activator inhibitor-1. References are in parentheses.
*
Experimental data from the current study.