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Schematic overview of in situ NETosis assay. Pneumonic bronchoalveolar lavage (BAL) is evaluated for neutrophil extracellular trap (NET) content using an ELISA-based method. This assay utilizes an anti-DNA capture antibody, which binds NETs, and an anti-citrullinated histone H3 (H3Cit) antibody to detect this NET-specific protein. A horseradish peroxidase (HRP)-conjugated secondary antibody against the H3Cit antibody is used to generate a measurable assay signal.
