Fig. 2.

T-lymphocytes are activated, necessary, and sufficient to confer mixed-granulocytic airway inflammation in the complete Freund’s adjuvant and house dust mite antigen (CFA/HDM) model. Wild-type mice were administered saline during the challenge or subjected to the CFA/HDM sensitization and challenge model. Lung total cells (A), neutrophils (B and C), total and activated CD8⁺ T cells (D–F), and total and activated CD4⁺ T cells (G–I) were enumerated. n = 4 mice/group. *P ≤ 0.05 and **P ≤ 0.01 compared with saline. Wild-type mice were naive or subjected to the CFA/HDM model without or with administration of a lymphocyte-depleting anti-CD90 antibody during the challenge phase. Total cells (J), macrophages (K), lymphocytes (L), eosinophils (M), and neutrophils (N) were measured from BAL. n = 4 mice/group. *P ≤ 0.05 and **P ≤ 0.01 compared with naive; #P ≤ 0.05 compared with CFA/HDM. CD4⁺ T cells enriched from naive mice or mice subjected to the CFA/HDM model were administered by adoptive transfer (AT) to naive recipients that were subsequently challenged with intranasal HDM. BAL cells (O) and cytokines (P) were measured after the HDM challenge. n = 4 mice/group. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 compared with AT naive.