Fig. 8.
Combined IL-17A neutralization and corticosteroid administration decreases inflammation and methacholine hyperresponsiveness in the complete Freund’s adjuvant and house dust mite antigen (CFA/HDM) model of severe asthma. Wild-type mice were naive or subjected to the CFA/HDM model without or with the administration of IL-17A-neutralizing antibody without or with 2.5 mg/kg dexamethasone (Dex) during the HDM challenge. Total cells (A), macrophages (B), lymphocytes (C), eosinophils (D), and neutrophils (E) were measured from BAL. Airway resistance (RN; F), tissue damping (G; G), and tissue elastance H; H) were measured. Body weight was measured on the day preceding the HDM challenge (day 0), throughout the HDM challenge period (days 1–4), and immediately preceding assessment of lung function and BAL on day 5 (I). n= 8 mice/group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001 compared with naive or to group indicated by brackets (A–H). **P ≤ 0.01 compared with CFA/HDM; #P ≤ 0.05, ##P ≤ 0.01, and ###P ≤ 0.001 compared with CFA/HDM + antiIL-17A (I).