Table 4. Comparison of giant sequoia v2.0 assembly and annotation to selected gymnosperm genome projects.

A	Sequoiadendron giganteum	Abies alba	Picea glauca	Pinus lambertiana	Pinus taeda	Pseudotsuga menzesii	Ginkgo biloba	Gnetum montanum
Reference		Mosca et al., 2019	Warren et al., 2015	Stevens et al., 2016	Neale et al., 2014	Neale et al., 2017	Guan et al., 2016	Wan et al., 2018
Genome size (Mbp)	8,114	18,167	20,000	31,000	20,613	15,700	10,610	4,110
Chromosomes	11	12	12	12	12	12	12	22
TE content (%)	79	78	N/A	79	81	72	77	86
N50 scaffold size (kb)	690,549	14.05	71.50	246	107	340	1,360	475

B	Sequoiadendron giganteum	Abies alba	Picea glauca	Pinus lambertiana	Pinus taeda	Pseudotsuga menzesii	Ginkgo biloba	Gnetum montanum
Number of genes	37,936	94,209	14,462	38,518	51,751	46,688	41,840	27,493
Average overall CDS size (bp)	1,084	629	1,421	1,102	1,131	1,180	1,186	1,290
Average size introns (bp)	4,067	315	603	11,468	5,596	4,685	7,884	1,769
Maximum intron size length (kb)	1,399.11	36.01	119.32	1,254.69	758.52	351.90	1,272.92	342.13

Assembly (A) and annotation (B) statistics for giant sequoia v2.0 compared to recent gymnosperm genome projects. A Genome size, TE content, and N50 scaffold size are as reported in the literature. B Number of genes, average coding sequence (CDS) size, average intron size, and maximum intron length as calculated by gFACs.