Figure 4.

Differential and shared locations of 1.688 arrays in heterochromatin visualized in nine Drosophila species from the melanogaster group. FISH was performed on neuroblast chromosome spreads from D. melanogaster, D. simulans, D. sechellia, D. erecta, D. yakuba, D. eugracilis. D. biarmipes, D suzukii and D. takahashii (A-I, respectively). The panels show species-specific probes for 1.688 satDNA (red) labeled with Bio-16-dUTP combined with DAPI staining (gray). The X and Y sex chromosomes and autosomal labels (when known) are identified in each panel. Note that the X chromosome from all species except D. simulans has a clear signal. Bar = 5 µm. White arrows indicate signals of hybridization.