Table 2. Primers for the construction of dual-luciferase reporter plasmid.
| Genes | Primers (5′-3′) | Products (bp) |
|---|---|---|
| ATP8A1 | ATP8A1_WT _F: GCGGCTCGAGAGCACTTGTAGTTCTGATG | 687 |
| ATP8A1_WT _R: AATGCGGCCGCACATAGCGACCACTTTCTG | ||
| ATP8A1_mut_F: TAGAACTGTTCGGTATAATGCACTAATATTGTTT | ||
| ATP8A1_mut_R: AGTGCATTATACCGAACAGTTCTACAATAAAGAA | ||
| CPLX1 | CPLX1_WT _F: GCGGCTCGAGTGAACAAGACCAAAGATAA | 781 |
| CPLX1_WT- R _R: AATGCGGCCGCACAGAGAGAGAAAACAAGC | ||
| CPLX1_mut_F: TTTAGAGATTCGGTATTGAAATCAGACTGCAATA | ||
| CPLX1_mut_R: TGATTTCAATACCGAATCTCTAAAGATAGGTATG | ||
| KLF4 | KLF4_WT _F: GCGGCTCGAGATCACCTCGCCTTACACAT | 1033 |
| KLF4_WT _R: AATGCGGCCGCTCAACCACTGACCAACATT | ||
| KLF4_mut_F: CCTTCTAATTCGGTATACAATGTTAAAAGAGGA | ||
| KLF4_mut_R: ACATTGTATACCGAATTAGAAGGAAGAAAAAAA |
Note: WT, wild type; MUT, mutant type.