Table 1. Table of the oligonucleotides used. These include the bridge primers for adding the sgRNA sequences to the pKOPIS + sgRNA plasmid, the primers for PCR SOEing to clone the donor DNA, and the primers for PCR and Sanger sequencing.

Name	Sequence
ADE2 Bridge L1	cgggtggcgaatgggactttACAGTTGGTATATTAGGAGGgttttagagctagaaatagc
ADE2 Bridge L2	cgggtggcgaatgggactttAACAGTTGGTATATTAGGAGgttttagagctagaaatagc
ADE2 Bridge H1	cgggtggcgaatgggactttACTTTGGCATACGATGGAAGgttttagagctagaaatagc
ADE2 Bridge H2	cgggtggcgaatgggactttACGGAGTCCGGAACTCTAGCgttttagagctagaaatagc
ADE2 5′ KO For	gatgtccacgacgtctctCAAATGACTCTTGTTGCATGG
ADE2 5′ KO Rev	GTATATCAATAAACTTATATAACTTGATTGTTTTGTCCGATTTTC
ADE2 3′ KO For	GAAAATCGGACAAAACAATCAAGTTATATAAGTTTATTGATATAC
ADE2 3′ KO Rev	cggtgtcggtgtcgtagGTATAATAAGTGATCTTATGTATG
ADE2 Conf For	ACCAACATAACACTGACATC
ADE2 Conf Rev	TATATGAACTGTATCGAAAC
pKOPIS sgRNA For	AACGCGAGCTGCGCACATAC
pKOPIS sgRNA Rev	GCGACAGTCACATCATGCC
pKOPIS sgRNA Seq For	CACCTATATCTGCGTGTTG
pKOPIS sgRNA Seq Rev	GCACGTCAAGACTGTCAAGG